Evidence of Piezoelectric Resonance in Isolated Outer Hair Cells
نویسندگان
چکیده
منابع مشابه
Acetylcholine-induced phosphorylation in isolated outer hair cells.
Two groups of isolated, surviving outer hair cells (OHCs) of guinea pig cochleas (n = 20, for each group) were treated with 10 microM acetylcholine or acetylcholine plus strichnine (an alpha9 nAChR antagonist), respectively, under short-term tissue culture conditions. The protein content of the cell homogenates was separated by SDS-polyacrylamide gel electrophoresis, Western blotted and labelle...
متن کاملFurosemide alters nonlinear capacitance in isolated outer hair cells.
The outer hair cell (OHC) from the organ of Corti plays a crucial role in hearing through its unique voltage-dependent mechanical responses. Furosemide, one of the loop diuretics, disrupts normal cochlear function. Here we report on direct effects of furosemide on OHC motility-related, voltage-dependent capacitance using the whole-cell patch-clamp technique. Extracellularly applied furosemide r...
متن کاملShape changes in isolated outer hair cells: with attached microspheres
Shape changes can be induced in isolated outer hair cells by various stimuli and quantified from digitized video-images. While overall changes in length between base and apex are easily measured, changes in defined segments of the cell require fixed landmarks on the cell body. The problem of locating such landmarks makes it difficult to assess if a change in length is uniform or largely confine...
متن کاملComparison of isolated outer hair cells from five mammalian species.
Live outer hair cells were isolated from guinea pig, chinchilla, rat, mouse, and gerbil. The organ of Corti from selected turns of the cochlea was briefly incubated with collagenase and outer hair cells were separated from the tissue by micromanipulation under microscopic observation. Morphological criteria for cell viability were: cylindrical cell shape without swelling or distortion of the me...
متن کاملEvidence that phosphoinositides mediate motility in cochlear outer hair cells.
Cochlear outer hair cells are postulated to modulate auditory transduction by their mechanical properties which presumably are controlled by efferent neurons and regulated by the levels of intracellular calcium. In a number of biological systems these calcium levels are controlled by inositol trisphosphate (InsP3), the second messenger of the phosphoinositide cascade. We have investigated wheth...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2005
ISSN: 0006-3495
DOI: 10.1529/biophysj.104.050872